FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24774775
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG22920309.251466461350304TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG331830.13393865332783042TruSeq Adapter, Index 1 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG276120.11145207171407209TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTC2794050.065.5796738
ACGATCT2789050.065.5646437
GATCTCG2799700.065.5513539
CACGATC2789800.065.5498836
CTCGTAT2903050.065.39973442
TCTCGTA2857700.065.38267541
TCACGAT2852400.065.363135
TCGTATG2965100.065.3073143
ATGCCGT2960400.065.2959847
CGTATGC2971800.065.2756844
TATGCCG2967550.065.2262246
ATCTCGT2818150.065.20946540
ATCACGA2907750.065.19041434
CCGTCTT2952450.065.1865950
TGCCGTC2961150.065.1632348
GCCGTCT2953750.065.1589449
CGTCTTC2956400.065.081251
CATCACG2968250.065.0352333
GTATGCC2997850.064.6163245
CGTCTGA3070150.064.6130916
Produced by FastQC (version 0.11.2)