FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28947467
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT45100.026.31311242
TCGTATG46600.025.98455443
TCTCGTA46250.025.28528241
CGTATGC50100.024.03213144
TATGCCG52250.023.04404646
ATGCCGT52750.022.5642447
AATCTCG54250.021.6834939
ATCTCGT56700.020.68603340
TGCCGTC66350.018.04359848
CCGTCTT68750.017.31334550
GCCGTCT76850.015.53341149
CGTCTTC80800.014.98746351
GTATGCC86750.014.27717645
GTAATCT96900.012.49549437
TAATCTC126350.09.93798838
GCTTGAA131350.09.8768259
TGTAATC129150.09.6958236
TTGTAAT137750.09.19075835
TGCTTGA156400.08.44921158
CTTGTAA152950.08.39040834
Produced by FastQC (version 0.11.2)