FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25987563
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT44900.026.8910542
TCGTATG45000.026.75483743
TCTCGTA45800.026.43758641
CGTATGC47800.025.83764344
TATGCCG49650.025.01440446
ATGCCGT51400.023.82742947
ATCTCGT55150.022.08052440
TGCCGTC60050.020.56811548
CCGTCTT61100.020.27131550
GCCGTCT68000.017.9605549
CATCTCG67050.017.95564539
CGTCTTC71500.017.41919951
GTATGCC82350.015.45849445
GATCATC104250.012.04466336
GCTTGAA121700.010.74500359
CGCGTAT5959.145879E-99.85639342
AGATCAT139650.09.26331335
TGCTTGA145100.09.22593858
TCGCGTA5301.8375502E-58.46150741
ATCATCT170200.07.499155537
Produced by FastQC (version 0.11.2)