FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27551816
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39150.015.33206942
TCGTATG39400.015.05972843
TATGCCG41900.014.73822646
TCTCGTA40500.014.65045841
CGTATGC41850.014.17840344
ATGCCGT45150.013.21901947
TATCTCG45950.012.23700539
ATCTCGT52500.011.4332640
TGCCGTC53950.011.38300148
CCGTCTT58850.010.61121450
GCCGTCT59400.010.16446749
CGTCTTC67650.09.33285851
CGCCAAT63600.09.0040433
GTATGCC79000.08.07884445
ACGCCAA79950.07.076417432
GCTTGAA116850.06.05339659
TCACGCC103950.05.940746330
GTCACGC102100.05.91350929
GCCAATA102600.05.884050434
CAATATC105950.05.66514636
Produced by FastQC (version 0.11.2)