FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27914477
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34000.016.64025343
CTCGTAT33000.016.5169742
TCTCGTA33500.015.96128741
CGTATGC36850.015.16610544
ATGCCGT38950.014.08344147
TATGCCG40750.013.9690546
ATCTCGT43700.012.63051540
TGCCGTC49200.011.57049548
GATCTCG48850.011.29874439
CCGTCTT51950.011.02437750
GCCGTCT54650.010.85851949
CGTCTTC61350.09.44765351
GTATGCC75400.08.09860345
AGTGATC107150.05.537386436
GCTTGAA114500.05.454405359
CGTCTGA360950.05.240266369
ACGTCTG396000.05.06408369
ACACGTC455100.05.020777769
Produced by FastQC (version 0.11.2)