FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31735195
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG373750.1177714521684836TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT87100.036.8337242
TCGTATG89850.036.55166643
TCTCGTA90850.035.1610141
CGTATGC93850.035.10445444
TATGCCG93850.035.0322646
ATGCCGT96750.034.1970147
AATCTCG97300.032.72321739
ATCTCGT102900.030.90942240
CCGTCTT111900.029.53745350
TGCCGTC115750.028.82316848
GCCGTCT121950.026.99003849
CGTCTTC125600.026.39801851
GTATGCC133800.024.75250445
GACCAAT162900.020.05373835
ACCAATC163250.019.67240336
GCTTGAA178150.018.94240659
CCAATCT190550.017.10736537
CAATCTC190200.017.01202438
TGCTTGA202500.016.7492358
ACTGACC233700.014.39227532
Produced by FastQC (version 0.11.2)