FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27176681
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG70700.015.66282443
CTCGTAT69450.015.39819942
ATGCCGT70700.015.32264947
TATGCCG72750.014.9380846
TCTCGTA70900.014.93720741
CGTATGC74700.014.91681144
TATCTCG76650.013.59152139
ATCTCGT81150.012.92301840
CCGTCTT84300.012.64662250
TGCCGTC87900.012.40311148
CGTCTTC93700.011.74601851
GCCGTCT93400.011.59909249
CGATGTA96650.011.1004134
GTATGCC107750.010.34181845
CCGATGT112850.09.7208933
ACCGATG116000.09.45698732
GCTTGAA126400.09.30860259
CACCGAT121850.09.03135331
TGCTTGA144050.08.191758
GTATCTC134100.08.12883738
Produced by FastQC (version 0.11.2)