FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29041232
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG21798757.506138169344882TruSeq Adapter, Index 16 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG631080.21730483059396377RNA PCR Primer, Index 16 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2912750.065.6059941
CTCGTAT3018300.065.5202242
CGTCCAT2994000.065.4125835
ATCTCGT2892100.065.3792440
TCGTATG3106600.065.3159643
CATCTCG2889300.065.2848439
ATGCCGT3123250.065.2746847
CGTATGC3146850.065.2500444
TGCCGTC3118700.065.18851548
CCGTCCA3118900.065.18732534
CGTCTGA3293600.065.1629916
GCCGTCT3085500.065.1612349
CCGTCTT3083400.065.1553750
ACCCGTC3189150.065.12926532
TATGCCG3146000.065.1171546
ACGTCTG3301600.065.08543415
CGTCTTC3082500.065.0318151
TCACCCG3199300.064.9915430
CACCCGT3200650.064.9327931
CACGTCT3315350.064.8954314
Produced by FastQC (version 0.11.2)