FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21697984
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG13523806.232744940728134TruSeq Adapter, Index 15 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG218670.10077894794281349RNA PCR Primer, Index 15 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1811450.065.13622
GATCGGA1776750.065.1049961
AATCTCG1654650.065.0059139
TCGGAAG1807150.064.8871843
TCTCGTA1674400.064.8026541
CTCGTAT1688500.064.7952142
ACACGTC1785000.064.7692613
CGTCTGA1779050.064.76214616
CGGAAGA1805050.064.70894
ACGTCTG1782300.064.6827615
CGTATGC1738850.064.6364644
TCGTATG1737250.064.6355243
ATGCCGT1733450.064.595147
ATCTCGT1674850.064.5217440
TATGCCG1738750.064.5177646
TGCCGTC1734350.064.4916148
CACGTCT1790700.064.46113614
GCCGTCT1732300.064.4568449
CCGTCTT1733300.064.4334450
CGTCTTC1737350.064.2849351
Produced by FastQC (version 0.11.2)