FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22985885
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG13910756.051866177873943TruSeq Adapter, Index 14 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1769650.064.86050441
CTCGTAT1806500.064.84843442
TCGTATG1841500.064.7250843
ATGCCGT1843800.064.7215447
CGTATGC1854200.064.66601644
GCCGTCT1832100.064.6521649
TGCCGTC1842800.064.645648
ATCTCGT1760250.064.59778640
CCGTCTT1833500.064.5822550
TATGCCG1854100.064.567746
CATCTCG1757750.064.4454539
CGTCTTC1837950.064.3783951
CGTCTGA1924950.064.29700516
ACACGTC1933400.064.2929313
ACGTCTG1930500.064.192715
GATCGGA1931300.064.172241
ATCGGAA1977100.064.042292
CACGTCT1939850.064.0118614
TCGGAAG1980300.063.7892043
GTATGCC1879050.063.76434745
Produced by FastQC (version 0.11.2)