FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28706929
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG14962155.212034348919733TruSeq Adapter, Index 13 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1928850.063.6382542
TCTCGTA1918550.063.61837441
AATCTCG1908200.063.5408839
ATGCCGT1978650.063.45320547
TCGTATG1986200.063.41483743
CGTATGC1987950.063.38788244
ATCTCGT1921200.063.32750340
TATGCCG1986150.063.30998646
TGCCGTC1982000.063.2688448
GCCGTCT1977500.063.2044449
CCGTCTT1979300.063.1386950
CGTCTGA2080050.063.0996616
CGTCTTC1983750.062.99324451
ACGTCTG2089950.062.86875515
ACACGTC2106100.062.62951713
CACGTCT2109200.062.4394514
GTATGCC2019650.062.32113645
CACACGT2141300.061.61930512
GCACACG2145950.061.52897311
GTCAAAT2024200.061.3415535
Produced by FastQC (version 0.11.2)