FastQCFastQC Report
Tue 8 May 2018
HWTTCBGX5_n01_mb100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTTCBGX5_n01_mb100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21858914
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2283161.0444983680342035TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC317050.061.5550344
TCGTATG317400.061.48616843
TCTCGTA310250.061.41203741
CTCGTAT313900.061.36945342
ATGCCGT318050.061.2228647
TATGCCG322550.060.57111446
TGCCGTC323400.060.1472848
ATCTCGT315050.060.02784340
CCGTCTT323500.059.872650
GCCGTCT323700.059.8462649
ACGGCTA332550.059.0160632
CGGCTAC329750.058.9730333
CATCTCG320400.058.89522639
CGTCTTC332150.058.31323651
TCACGGC344600.057.00517330
GTCACGG349650.056.46953629
GTATGCC348300.056.1521145
CACGGCT353750.055.59679831
AGTCACG359450.055.132428
CGTCTGA387300.052.03426416
Produced by FastQC (version 0.11.2)