FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_LA_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_LA_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16431898
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC646220.3932716719638839Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG435940.265301062603967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400960.2440131992055939No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC374870.2281355446583225No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA340730.20735888209627396No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG338360.20591656545092965No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG225490.1372269959319368No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT187110.11386998629129756No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG177330.10791814798266153No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC174350.1061046021585577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)