FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AJ_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AJ_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984960
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG985200.4694790935984629No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA814800.3882780810637714No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC541770.2581706136204215Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349800.1666908109426942No Hit
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT315410.1503028835890085No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG276030.13153706273445362No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)