FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AJ_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AJ_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13657100
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3482412.5498898009094173No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1868151.3678965519766275Illumina Single End PCR Primer 1 (100% over 50bp)
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT674220.493677281414063No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG592250.4336572185895981No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG250620.18350894406572404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239100.1750737711520015No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG204140.14947536446244078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG197410.14454752473072613No Hit
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC167310.12250770661414209No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT143400.10500032949894195No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC142750.10452438658280308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG137770.10087793162530845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)