FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AJ_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AJ_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405373
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3435662.5628977276499505No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2473351.8450437746118664Illumina Single End PCR Primer 1 (100% over 50bp)
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT611360.4560559411513577No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG519520.3875460981205074No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG437310.32621994180989966No Hit
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC317010.2364798055227557No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGGGG270440.20174000380295273No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT254730.19002082224791508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG230920.17225928737678542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGG230520.1719608995587068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG212540.15854836713607298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGGGGG202270.1508872599069045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179110.13361060524015259No Hit
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT170920.12750111466499292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG164260.12253295749398395No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC142610.10638271684047881No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGGGGGG136630.1019218189602035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)