FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AJ_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AJ_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18116984
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5478013.023687607164636Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4657152.5705989473744637No Hit
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT952580.5257939180163762No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG863280.4765031530634459No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC384090.21200548612285577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTGGGG362310.19998361758226424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTGGG307630.1698019935326984No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG294770.16270368180487438No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG275950.15231563929183797No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTGGGGG273280.15084188405752305No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG250270.13814109456629206No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA207960.11478731780079951Illumina Single End PCR Primer 1 (100% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG206500.113981444152073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTGGGGGG183260.10115370196275494No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)