FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AJ_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AJ_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13100295
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTATATATACATGTATATATACATGTATATATACATGTATATATACA6328274.8306316766149155No Hit
ATATATACATGTATATATACATGTATATATACATGTATATATACATGTAT4876573.7224886920485383No Hit
ATATACATGTATATATACATGTATATATACATGTATATATACATGTATAT3703672.8271653424598453No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2322051.7725173364416602No Hit
ACATGTATATATACATGTATATATACATGTATATATACATGTATATATAC2245461.7140530041499065No Hit
ATATACATGTATATATACATGTATATAGATCGGAAGAGCGTCGTGTAGGG1948511.487378719334183Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATACATGTATATATACATGTATATATAGATCGGAAGAGCGTCGTGT1531771.1692637455874086No Hit
ATACATGTATATATACATGTATATATACATGTATATATACATGTATATAT1494781.1410277402150104No Hit
ATATATTCATGTATATATACATGTATATATACATGTATATATACATGTAT1305090.9962294742217637No Hit
CATGTATATATACATGTATATATACATGAGATCGGAAGAGCGTCGTGTAG1292660.9867411382720771Illumina Single End PCR Primer 1 (100% over 22bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1287870.9830847320613771Illumina Single End PCR Primer 1 (100% over 50bp)
TACATGTATATATACATGTATATATACATGTATATATACATGTATATATA1190660.9088802962070701No Hit
TATATACATGTATATATACATGTATATATACATGTATATATACATGTATA1171690.8943997062661566No Hit
ATGTTTATATACATGTATATATACATGTATATATACATGTATATATACAT1111360.8483473082094717No Hit
CATGTTTATATACATGTATATATACATGTATATATACATGTATATATACA1024580.782104525127106No Hit
TATACATGTATATATACATGTATATATACATGTATATATACATGTATATA1008280.7696620572284822No Hit
ACATGTTTATATACATGTATATATACATGTATATATACATGTATATATAC971740.7417695555710768No Hit
ATGTATATATACATGTATATATACATGTATATATACATGTATATATACAT944520.7209913975219642No Hit
TATATATACATGTATATATACATGTATATATACATGTATATATACATGTA880520.6721375358341167No Hit
ATATATATGTATATATACATGTATATATACATGTATATATACATGTATAT833300.6360925460075517No Hit
ATATATACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG782680.5974521947788198Illumina Single End PCR Primer 1 (100% over 32bp)
ATATTCATGTATATATACATGTATATATACATGTATATATACATGTATAT751230.5734451018087761No Hit
ATACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA739640.5645979727937425Illumina Single End PCR Primer 1 (100% over 36bp)
GTATATATACATGTATATATACATGTATATATACATGTATATATACATGT733310.5597660205361787No Hit
TGTATATATACATGTATATATACATGTATATATACATGTATATATACATG728370.5559951130871481No Hit
ATATATACATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA709390.5415068897303458Illumina Single End PCR Primer 1 (100% over 36bp)
ATATACATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA669820.5113014630586563Illumina Single End PCR Primer 1 (100% over 36bp)
ACATGTATATATACATGTATATATACATGTAGATCGGAAGAGCGTCGTGT634720.48450817328922746No Hit
ATATATGTATATATACATGTATATATACATGTATATATACATGTATATAT608810.464729992721538No Hit
ATATATATATGTATATATACATGTATATATACATGTATATATACATGTAT532420.40641832874755873No Hit
ATGTATATATACATGTATATATACATAGATCGGAAGAGCGTCGTGTAGGG496200.37877009639859255Illumina Single End PCR Primer 1 (100% over 24bp)
ATATTTACATGTATATATACATGTATATATACATGTATATATACATGTAT467970.35722096334471853No Hit
ATATACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA463740.3539920284237874Illumina Single End PCR Primer 1 (100% over 34bp)
ACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC456360.3483585674979075Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATACATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC451940.34498459767509054Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATACATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA448370.3422594682028153Illumina Single End PCR Primer 1 (100% over 34bp)
ATACATGTATATATACATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG431830.3296337983228622Illumina Single End PCR Primer 1 (100% over 28bp)
GTATATATACATGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAG403250.3078174957128828Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATACATGTATATATACATGTATATATACATGTATATATAGATCGGA355500.27136793484421534No Hit
ATTTACATGTATATATACATGTATATATACATGTATATATACATGTATAT353770.2700473538954657No Hit
ATACATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC329880.2518111233372989Illumina Single End PCR Primer 1 (100% over 38bp)
ATATACATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC326780.24944476441179378Illumina Single End PCR Primer 1 (100% over 38bp)
CATGTATATATACATGTATATATACATGTATATATACATGAGATCGGAAG303530.23169707247050544No Hit
ATATACATGTATATATACATGTATATATACATGTATATAGATCGGAAGAG267840.20445341116364174No Hit
TTATACATGTATATATACATGTATATATACATGTATATATACATGTATAT237790.1815149964180196No Hit
TTATATACATGTATATATACATGTATATATACATGTATATATACATGTAT236520.18054555260015137No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG228530.17444645330505915No Hit
TATATATATATGTATATATACATGTATATATACATGTATATATACATGTA211910.16175971609799625No Hit
TTACATGTATATATACATGTATATATACATGTATATATACATGTATATAT187720.14329448306316767No Hit
TCATGTATATATACATGTATATATACATGTATATATACATGTATATATAC180690.13792819169339315No Hit
ATATACATGTATATTTACATGTATATATACATGTATATATACATGTATAT176270.1345542218705762No Hit
ATATATACATGTATATATTCATGTATATATACATGTATATATACATGTAT168230.12841695549604035No Hit
CATGTATATATACATGTATATATACATGTATATATACATGTATATATAGA164340.12544755671532587No Hit
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA163680.12494375126666994Illumina Single End PCR Primer 1 (100% over 34bp)
ATACATGTATTTATACATGTATATATACATGTATATATACATGTATATAT157930.12055453713065241No Hit
CATGTATTTATACATGTATATATACATGTATATATACATGTATATATACA157180.11998203093899794No Hit
ATACATGTATATATACATGTATATATACATGTATAGATCGGAAGAGCGTC156560.11950875915389692No Hit
ACATGTATATATACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAA152390.1163256247282981Illumina Single End PCR Primer 1 (100% over 26bp)
ATATACATGTTTATATACATGTATATATACATGTATATATACATGTATAT148960.11370736307846503No Hit
CATGTATATATACATGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAA144770.11050896182108877Illumina Single End PCR Primer 1 (100% over 27bp)
ATATATACATGTATATATACATGTAGATCGGAAGAGCGTCGTGTAGGGAA144740.11048606157342258Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATACATGTTTATATACATGTATATATACATGTATATATACATGTAT143860.10981432097521468No Hit
ACATGTATTTATACATGTATATATACATGTATATATACATGTATATATAC141590.10808153556847383No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC139820.10673042095616932No Hit
ACATGTATATATACATGTATATATACATGTATATATACATGTAGATCGGA139030.10612738110095996No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG139010.10611211426918249No Hit
ATGTATATATACATGTATATATACATGTATATATACATAGATCGGAAGAG138190.10548617416630693No Hit
ATATGCATGTATATATACATGTATATATACATGTATATATACATGTATAT136470.10417322663344604No Hit
ATATACATGTATATATTCATGTATATATACATGTATATATACATGTATAT134760.10286791251647386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG132130.10086032413773888No Hit
ATATATACATGTATATATACATGAGATCGGAAGAGCGTCGTGTAGGGAAA131930.10070765581996435Illumina Single End PCR Primer 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)