FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_AH_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_AH_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22625368
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC643680.28449482015054955Illumina Single End PCR Primer 1 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC413250.18264896288095733No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG365370.16148687614716367No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA355030.15691678473472784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336870.14889039594847694No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG297920.1316752063436051No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC253980.1122545277495597No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG229300.10134641787925835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)