FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n02_13G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n02_13G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29844737
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT835780.2800426755310325No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT541410.18140886951022556No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC502650.16842165504758846Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGG442160.14815342484003124No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG437390.14655515309114636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354790.1188785815066824No Hit
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA338020.1132595003266405No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA336080.11260946946860347No Hit
GCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTGA329930.1105488046351355No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC327660.10978820151774163No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG318280.10664526881238726No Hit
CGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG316770.10613931695896667No Hit
ATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACA312700.10477559242689925No Hit
GCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGC302630.10140146317925333No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGA300010.10052358645345073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)