FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27309688
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT2332690.8541620834335419TruSeq Adapter, Index 22 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA754660.276334171228906No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC690840.2529651748493062No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG683900.25042395211545443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCGCGTAT472090.17286539487378985TruSeq Adapter, Index 22 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG398000.14573582825259665No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388690.14232678161683868No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTA334640.12253527026745965TruSeq Adapter, Index 22 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT330690.12108889709761606No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC307020.11242164319123675No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG291920.10689246980778397No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC283540.10382396166517904No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG275640.10093121532549182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)