FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28057073
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGGTT1237270.4409832771935975TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTTT783740.27933776270960264TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGGAT487430.1737280292922929TruSeq Adapter, Index 9 (97% over 36bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA433380.154463724708561No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTAT403270.14373202792750334TruSeq Adapter, Index 9 (97% over 36bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC366030.13045908245667678No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGGGT357660.12747587747303507TruSeq Adapter, Index 9 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG357650.12747231330937478No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314040.11192899558695947No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)