FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28358843
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTAT1975370.6965622680727842TruSeq Adapter, Index 27 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA636010.22427219615412378No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG564560.19907723315792536No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC549490.1937631940767118No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCGCGTAT479130.16895259090788717TruSeq Adapter, Index 27 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG291830.1029061728646687No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT290370.10239134227020474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)