FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23154252
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGT2042430.882097162974645TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGTT1360480.5875724251424749TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCGCGGGT450720.19465971088161257TruSeq Adapter, Index 16 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA350000.15116014112656284No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG333730.14413335399476518No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC301200.13008409859234493No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGG245310.10594598348502038TruSeq Adapter, Index 16 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT238190.10287095432838858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)