FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27559498
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTTGCATCTCGTAT3902561.416049015116313TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTTGCATCGCGTAT814070.295386367342395TruSeq Adapter, Index 9 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA427940.1552785903429736No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTTGCATCTCGTA415290.1506885212495525TruSeq Adapter, Index 9 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG332110.1205065491396106No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC321760.11675103806317516No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)