FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45308740
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGTT3747310.8270611806905246TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTTT3060080.6753840429020979TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGAT2181420.48145677853765084TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTAT2143570.473102981897091TruSeq Adapter, Index 9 (97% over 36bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1327880.2930736983637152No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1100610.24291339816556365No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1053280.2324672899753999No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT896880.19794856356632295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGGT829880.18316112961870049TruSeq Adapter, Index 9 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT693920.15315367410349526No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGGTT647390.14288413228882552TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTGT621860.13724945783087325TruSeq Adapter, Index 9 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGT577910.12754934257717165TruSeq Adapter, Index 9 (97% over 36bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG561250.1238723478075091No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG558040.12316387522583942No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG534010.11786026272193842No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC466750.10301544470228038No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGTTT466410.10294040399269545TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)