FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22619979
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT1628580.7199741432120693TruSeq Adapter, Index 27 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA659020.29134421389162213No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC541460.23937245918751737No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG478320.21145908225644242No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT310510.13727245281704284No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCGCGTAT292600.1293546735830303TruSeq Adapter, Index 27 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG292020.12909826308857317No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG243150.10749346849526252No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC236090.10437233385583602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)