FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_LA_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_LA_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33864146
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT9303562.74731865377618TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT3169550.9359604107541941TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGAT3037020.8968246238957274TruSeq Adapter, Index 11 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2018220.5959754602995155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTAT1513980.44707461395896414TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGTT1245830.3678905707529137TruSeq Adapter, Index 11 (97% over 39bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1020940.3014811003944998No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC883100.2607772834430846No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG855030.2524882806730162No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTT821740.2426578245912358TruSeq Adapter, Index 11 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTA589140.17397160997356909TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGGAT575830.17004119932627268TruSeq Adapter, Index 11 (97% over 39bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG538410.15899116428331014No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGT512530.15134886318999452TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCGCGTTT488430.1442321917700213TruSeq Adapter, Index 11 (97% over 39bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT476650.14075358640374394No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTGT414350.12235654783675927TruSeq Adapter, Index 11 (97% over 39bp)
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC385720.11390217842788654No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG377840.1115752335818538No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG374030.11045014984284558No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA359240.10608269879299481No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC356950.1054064673593127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344170.10163256442374186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)