FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15922182
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTCGTAT6801444.27167582935555TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCGCGTAT3084561.9372721653351281TruSeq Adapter, Index 20 (97% over 37bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG568030.35675386702651685No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTCGTA425910.26749474412489443TruSeq Adapter, Index 20 (97% over 37bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA412020.25877106542306827No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT329370.20686235090140284No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCGCGTA321960.20220846615118457TruSeq Adapter, Index 20 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGATTGATCTCGTA211220.13265769729299665TruSeq Adapter, Index 18 (97% over 36bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG204660.12853765897161581No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183980.11554948938531162No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGTGATCGCGTAT177220.11130384013949847TruSeq Adapter, Index 20 (97% over 37bp)
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC160050.10052014227698187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)