FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13657100
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGTT3723752.72660374457242TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTTT3468332.5395801451259787TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTAT3118482.283413023262625TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGAT2694901.9732593303117059TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGGT875410.6409925972571044TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTGT724550.5305299075206303TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGTT666360.48792203322813776TruSeq Adapter, Index 10 (97% over 38bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG658850.4824230619970565No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGT652460.47774417702147604TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGTTT543350.39785166689853635TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGTAT543240.39777112271272813TruSeq Adapter, Index 10 (97% over 38bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA500340.3663588902475635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGAT482130.3530251663969657TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGTT407330.29825512004744786TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGGT344730.25241815612392093TruSeq Adapter, Index 10 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272720.19969100321444522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264150.19341587892012213No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG250960.18375789882185822No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGTGT243420.17823696099464748TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCGCGGT238910.1749346493765148TruSeq Adapter, Index 10 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTGCAATCTCGGT199880.1463561078120538TruSeq Adapter, Index 10 (97% over 35bp)
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC186330.13643452856023608No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT143240.10488317431958469No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)