FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405373
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTAT185467713.835325581764865TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGTAT3205062.3908771505276283TruSeq Adapter, Index 23 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTA1388461.0357488747235903TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGGAT1195200.8915828004189066TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTTT841490.6277259125874379TruSeq Adapter, Index 23 (97% over 38bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG577910.4311032598645334No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTA558030.41627338530602614TruSeq Adapter, Index 16 (97% over 36bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG433100.3230794100246222No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGTA427120.3186185121443469TruSeq Adapter, Index 23 (97% over 38bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA424380.3165745555905084No Hit
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC351970.2625589008228268No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGGAT289600.21603278028891848TruSeq Adapter, Index 23 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTT273290.20386601700676288TruSeq Adapter, Index 23 (97% over 38bp)
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT250430.18681315320356995No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191470.14283078881878183No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGTA158380.11814665656822829TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCGCGTTT152090.11345450812894203TruSeq Adapter, Index 23 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGTATG145250.10835207643979769TruSeq Adapter, Index 23 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCTTAAATCTCGGA138040.10297363601893061TruSeq Adapter, Index 23 (97% over 38bp)
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT135390.10099681672415979No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)