FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049407
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT158949415.816793966051929TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTAT2590342.5776048278271544TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTA1341141.3345464065690642TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTA654700.6514812267032274TruSeq Adapter, Index 9 (97% over 35bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG545470.5427882461124323No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTA445820.4436281663186693TruSeq Adapter, Index 7 (97% over 35bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA442960.44078222724982674No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTA203610.20260896986259985TruSeq Adapter, Index 9 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTATG169800.16896519366764626TruSeq Adapter, Index 7 (97% over 34bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA162220.16142246005162295No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG126270.12564920497298995No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGGAT115310.11474308882106178TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)