FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18116984
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT248750613.730243400336391TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTAT5183892.861342704723921TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTA3900452.1529245706680538TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTA1521760.8399632080041578TruSeq Adapter, Index 6 (97% over 37bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG920600.5081419732997502No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTA910030.5023076688702711TruSeq Adapter, Index 6 (97% over 35bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA712580.39332153740379744No Hit
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA343120.18939134681578346No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTA342460.18902704776909887TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTATG271750.14999737263111784TruSeq Adapter, Index 6 (97% over 36bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC215420.1189050009648405Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC185570.10242874862615102No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)