FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13100295
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATACATGTATATATACATGTATATATACATGTATATATACATGTAT8368556.388062253559938No Hit
CATGTATATATACATGTATATATACATGTATATATACATGTATATATACA7066945.394489208067451No Hit
ATATACATGTATATATACATGTATATATACATGTATATATACATGTATAT7012745.353116093950556No Hit
ACATGTATATATACATGTATATATACATGTATATATACATGTATATATAC4289263.274170543487761No Hit
ATACATGTATATATACATGTATATATACATGTATATATACATGTATATAT3381842.581499118912971No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGTT2407761.8379433440239321TruSeq Adapter, Index 10 (97% over 37bp)
ATGTATATATACATGTATATATACATGTATATATACATGTATATATACAT2389771.824210828840114No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTTT2279091.7397241817836928TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTAT2067661.5783308696483551TruSeq Adapter, Index 10 (97% over 37bp)
ATATACATGTATATATACATGTATATAGATCGGAAGAGCACACGTCTGAA1948381.4872794849276294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGAT1713451.3079476454537857TruSeq Adapter, Index 10 (97% over 37bp)
ATATATACATGTATATATACATGTATATATAGATCGGAAGAGCACACGTC1525561.1645233943205096No Hit
CATGTATATATACATGTATATATACATGAGATCGGAAGAGCACACGTCTG1306970.9976645564088442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATACATGTATATATACATGTATATATACATGTATATATACATGTA1273070.9717872765460625No Hit
GTATATATACATGTATATATACATGTATATATACATGTATATATACATGT1233540.941612383537928No Hit
TATATACATGTATATATACATGTATATATACATGTATATATACATGTATA1139060.8694918702212432No Hit
TACATGTATATATACATGTATATATACATGTATATATACATGTATATATA1017600.7767764008367751No Hit
TATACATGTATATATACATGTATATATACATGTATATATACATGTATATA977250.7459755677257649No Hit
TGTATATATACATGTATATATACATGTATATATACATGTATATATACATG802120.6122915552665036No Hit
ATATATACATGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC785390.5995208504846646Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATACATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT742330.5666513616678098TruSeq Adapter, Index 1 (97% over 35bp)
ATACATGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT708230.5406214134872536TruSeq Adapter, Index 1 (97% over 35bp)
ATATACATGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT668670.5104236202314528TruSeq Adapter, Index 1 (97% over 35bp)
ACATGTATATATACATGTATATATACATGTAGATCGGAAGAGCACACGTC633640.483683764373245No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGGT575190.43906644850364057TruSeq Adapter, Index 10 (97% over 37bp)
ATGTATATATACATGTATATATACATAGATCGGAAGAGCACACGTCTGAA515550.3935407561432777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTGT478700.3654116185933217TruSeq Adapter, Index 10 (97% over 37bp)
ATATATACATGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC465750.35552634501742136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGTT458920.3503127219654214TruSeq Adapter, Index 10 (97% over 37bp)
ATATATACATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGC457690.34937381181110805TruSeq Adapter, Index 10 (97% over 37bp)
ACATGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGC452970.34577083951162935TruSeq Adapter, Index 10 (97% over 37bp)
ATATACATGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC450540.34391591945066885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACATGTATATATACATGTATAGATCGGAAGAGCACACGTCTGAACTCC434760.33187038917825895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGGT430910.3289315240610994TruSeq Adapter, Index 10 (97% over 37bp)
GTATATATACATGTATATATACAGATCGGAAGAGCACACGTCTGAACTCC417380.31860351236365286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGTAT379590.28975683372015665TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGTTT376230.2871920059815447TruSeq Adapter, Index 10 (97% over 37bp)
ATATATACATGTATATATACATGTATATATACATGTATATATACATGAAT347860.26553600510522857No Hit
ATATATACATGTATATATACATGTATATATACATGTATATATAGATCGGA332250.253620242902927No Hit
ATATACATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGC330260.2521011931410705TruSeq Adapter, Index 10 (97% over 37bp)
ATACATGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGC325610.2485516547528128TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGAT322820.2464219317198582TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCTCGTT284390.21708671445948355TruSeq Adapter, Index 10 (97% over 37bp)
ATATACATGTATATATACATGTATATATACATGTATATAGATCGGAAGAG246190.18792706576454957No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGGT240260.18340045014253495TruSeq Adapter, Index 10 (97% over 37bp)
CATGTATATATACATGTATATATACATGTATATATACATGAGATCGGAAG218310.166645102266781No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA189060.144317360792257No Hit
ATATACATATGTATATATACATATGTATATATACATATGTATATATACAT183160.13981364541790853No Hit
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC171290.13075278075799057Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGTGT167420.12779864880905353TruSeq Adapter, Index 10 (97% over 37bp)
ATATACATATGTATATACATATGTATATACATATGTATATACATATGTAT167140.1275849131641692No Hit
ATATATACATATGTATATATACATATGTATATATACATATGTATATATAC167030.1275009455893932No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCGAGAATCGCGGT164700.12572235968732TruSeq Adapter, Index 10 (97% over 37bp)
ATATATACATGTATATATACATGTAGATCGGAAGAGCACACGTCTGAACT154920.11825687894814582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATACATGTATATATACATGTATATATACATGTATATATACATGAGA152210.11618822324230102No Hit
ACATGTATATATACATGTATATATAGATCGGAAGAGCACACGTCTGAACT148630.11345546035413706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACATGTATATATACATGTATATATACATGTATAGATCGGAAGAGCACA146960.1121806799007198No Hit
ATATATACATGTATATATACATGAGATCGGAAGAGCACACGTCTGAACTC146520.11184480960161583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG139330.10635638357762173No Hit
CATGTATATATACATGTATATATAGATCGGAAGAGCACACGTCTGAACTC137690.10510450337187062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)