FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_AJ_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_AJ_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21397001
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT554541025.916762821107497TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTAT9087144.246922267284093TruSeq Adapter, Index 7 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTA1376890.6434967218069486TruSeq Adapter, Index 1 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTA1270220.5936439410364097TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTATG1029930.48134315645449566TruSeq Adapter, Index 7 (97% over 36bp)
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC761530.3559050167824921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA677840.31679205884974254No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGGAT651610.3045333315636149TruSeq Adapter, Index 7 (97% over 37bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG455180.2127307467060454No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTA448980.20983314437383072TruSeq Adapter, Index 1 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTA426690.19941579663430403TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTATG362670.169495715778113TruSeq Adapter, Index 7 (97% over 36bp)
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC358830.1677010717529994No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTTT224950.10513155558575708TruSeq Adapter, Index 7 (97% over 37bp)
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT218340.10204233761544435No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTATGCC214980.10047202409346992TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)