FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_9G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_9G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28740005
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTAT1705170.593308873815436TruSeq Adapter, Index 3 (97% over 38bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1334320.4642727097646643No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1210400.42115511114211707No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1000970.3482845601453444No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG703810.24488861432000447No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG664300.23114122631502673No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT574490.1998921016193282No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC538580.18739732299976983No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG498460.17343768729337383No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC491640.1710646884021071No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC484690.1686464563941447No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT454400.1581071402040466No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG447550.15572370290123472No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA446960.15551841414084652No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC420250.14622474839513772No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT403680.1404592657516935No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT385470.13412314994378044No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTA370030.12875084746853732TruSeq Adapter, Index 3 (97% over 38bp)
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA368480.128211529538704No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG364020.12665968568898997No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT350210.12185453690770061No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCGCGTAT349560.1216283713242221TruSeq Adapter, Index 3 (97% over 38bp)
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT340840.11859427303509515No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG335070.11658661854790908No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCAT321360.11181626447177026No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAA320790.11161793465241221No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG314150.10930756623041646No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC311620.10842726019010783No Hit
TGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCA304910.1060925354745067No Hit
CGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGAC292250.10168752580244854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)