FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_8G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_8G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24515341
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTAT1424800.5811871023943742TruSeq Adapter, Index 7 (97% over 37bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT475630.19401320993250717No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT430050.17542077020262536No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTA307810.12555811481471948TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGTAT302960.12357976174999971TruSeq Adapter, Index 7 (97% over 37bp)
GGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGG287310.11719600392260503No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG281000.11462210539922738No Hit
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCG273370.11150976851596721No Hit
CCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCAC272750.11125686564996179No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG261620.10671685129731626No Hit
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA253670.10347398390256943No Hit
ATCGATTCTAGCATCACTGGTGGACAGCAAATCGGTCGGGATCTGTAGGA253600.10344543035318171No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC248320.10129167691365175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)