FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_7R_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_7R_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19584598
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT6740773.441873047381417TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGGAT1421340.7257437706916425TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTTT1149920.5871552737513428TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTA1085070.5540425185137832TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGTAT919310.4694045800684803TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTT759220.3876617738081731TruSeq Adapter, Index 22 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA445260.2273521264005521No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG441480.2254220382772217No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGGA337700.17243141779065366TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGGT316350.16152999413110242TruSeq Adapter, Index 22 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC313470.16005945079904116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGGTT297040.15167020533176123TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGTA270810.13827702769288397TruSeq Adapter, Index 22 (97% over 37bp)
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG253440.12940781322138958No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG252690.1290248592286653No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGGAT243930.12455195659364568TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGTT235660.12032925056720592TruSeq Adapter, Index 22 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG229160.11701031596359547No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT224870.11481981912521257No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC219550.11210339880348832No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC207900.10615484678317114No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)