FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_6R_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_6R_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19281673
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTAT10675975.536848384473691TruSeq Adapter, Index 18 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCGCGTAT1880380.9752162066019895TruSeq Adapter, Index 18 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTA1448350.7511536991629305TruSeq Adapter, Index 18 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCGCGTA492680.25551724686960514TruSeq Adapter, Index 18 (97% over 38bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA479150.2485002209092541No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG427490.2217079399697319No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGGAT419740.21768857920160767TruSeq Adapter, Index 18 (97% over 38bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG351370.18223003781881375No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG300760.15598231543497287No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTTT295440.15322321875285408TruSeq Adapter, Index 18 (97% over 38bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC285360.14799545661831315No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG231530.12007775466371616No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC230070.11932055895772115No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT228070.11828330456594716No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC219510.11384385576915447No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTT209880.1088494758727627TruSeq Adapter, Index 18 (97% over 38bp)
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCT205980.10682682980880341No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG205200.10642230059601157No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT201180.10433741926854584No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)