FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_5G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_5G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27161675
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGTT1137250.41869656418464624TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTTT993360.3657211861934141TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGTAT829890.30553712169812797TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGAT774000.28496033473635185TruSeq Adapter, Index 18 (97% over 37bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT445970.16419090501598302No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT407200.1499171166726647No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA351690.12948023271760672No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGGT338730.124708803856905TruSeq Adapter, Index 18 (97% over 37bp)
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG298010.10971709218963854No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)