FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_3G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_3G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24121638
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT7721743.2011673502437934TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTTT1385960.5745712625320055TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCGCGTAT1345070.5576196774033338TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGGAT1199270.4971760209650771TruSeq Adapter, Index 15 (97% over 36bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT403760.16738498438621788No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT334220.13855609639776537No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCGCGGAT266700.11056463080989774TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGGTT260320.10791970263379295TruSeq Adapter, Index 15 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)