FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_2G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_2G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24963856
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT2366820.9480987232100682TruSeq Adapter, Index 4 (97% over 38bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT522090.20913836388096455No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT447380.179211096234492No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTAT406050.1626551603245909TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTA329110.13183460119302082TruSeq Adapter, Index 4 (97% over 38bp)
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG307820.1233062712747582No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG258370.10349763273750658No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA254500.10194739146067819No Hit
GGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGG253600.10158687023350879No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG252240.1010420826013417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)