FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_14R_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_14R_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24580620
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTAT992140.4036269223477683TruSeq Adapter, Index 13 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA900680.3664187477777208No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG793990.32301463510684436No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC654580.26629922272098916No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT458400.18648837986999514No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG449540.18288391423812744No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG426290.17342524313870034No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG380980.1549920221703114No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC365510.14869844617426248No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT350480.1425838729861167No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC338700.13779147962907362No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC335530.13650184576304422No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC329630.1341015808388885No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG316500.12875997432123357No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT301710.12274303902830767No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA300800.1223728286755989No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCAT283100.11517203390313181No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC270690.11012334107113654No Hit
GGTACATCCGCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATT256750.10445220665711442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)