FastQCFastQC Report
Thu 28 Mar 2024
HWTKNDRX3_n01_10G_1-17_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTKNDRX3_n01_10G_1-17_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37162521
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT2846360.7659222042551957TruSeq Adapter, Index 2 (97% over 36bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT730530.19657708366986193No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT686720.18478832477484508No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTAT624450.16803219566293687TruSeq Adapter, Index 2 (97% over 36bp)
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG463750.1247897041215261No Hit
GGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGG462100.12434570840874869No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA433040.11652600209765103No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG410400.11043384274172358No Hit
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG372410.10021117781541247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)