FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n02_CITE-seq_test_TCells_300D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n02_CITE-seq_test_TCells_300D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25818527
Sequences flagged as poor quality0
Sequence length90
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT522130.20223074693610524Clontech SMART CDS Primer II A (100% over 26bp)
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC463470.17951062816248192No Hit
GTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGT402440.15587256391505216No Hit
CCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAG395010.15299478548873063No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC378440.14657691354739177No Hit
CATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTAT375990.14562798257235976No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA375130.14529488843418528No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA370970.14368364237045747No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA369290.14303294684472123No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC364630.14122804139833384No Hit
CAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTT346000.13401229280043744No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT326820.1265835188816155No Hit
GGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGAT326180.12633563487181124No Hit
GGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTA319030.12356630569977907No Hit
CCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTAC317330.12290786379873647No Hit
CTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGC311380.12060331714508733No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT310190.12014240781435749No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC307390.11905791527146378No Hit
AGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATG305720.11841109293338076No Hit
GTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAA305580.11835686830623607No Hit
GCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTC301580.11680759324495932No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA299430.11597485789952308No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA293590.11371291631005905No Hit
GGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGT290350.1124580035104249No Hit
TAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCA290070.11234955425613552No Hit
CAATTTCTATCGCCTATACTTTATTTGGGTAAATGGTTTGGCTAAGGTTG280060.1084724934152905No Hit
CAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCC277620.10752743562791169No Hit
GCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAG276990.10728342480576061No Hit
ACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTT275490.10670244665778182No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA269830.10451022244607525No Hit
GGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATC269020.10419649424616671No Hit
CCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGA266770.10332502702419856No Hit
GTTTATTGCTAAAGGTTAATCACTGCTGTTTCCCGTGGGGGTGTGGCTAG266740.10331340746123899No Hit
GTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTT266130.10307714301439427No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT265530.10284475175520277No Hit
ATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGG264670.10251165761702827No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT263380.10201201640976651No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT262880.10181835702710693No Hit
CTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT262160.10153948751607711No Hit
GCCTATACTTTATTTGGGTAAATGGTTTGGCTAAGGTTGTTTGGTAGTAA261430.1012567448173941No Hit
GCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAG259230.10040464353369191No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG258480.10011415445970251No Hit
TAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTT258340.10005992983255785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)