FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n02_CITE-seq_test_TCells_253D28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n02_CITE-seq_test_TCells_253D28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271898
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT669431.2698083308895582Clontech SMART CDS Primer II A (100% over 26bp)
GGAAGAGCGTCGTGTAGATCGGAAGAGCGTCGTGTAGATCGGAAGAGCGT71440.13551096777669067Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCGTCGTGTAGATCGGAAGAGCGTCGTGTAGATCGGAAGA62810.1191411518204639Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)