FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n02_CITE-seq_test_GEX_029D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n02_CITE-seq_test_GEX_029D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129664180
Sequences flagged as poor quality0
Sequence length90
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4650549235.8661058127233Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108073588.33488323452167Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60366394.655594937630424Clontech SMART CDS Primer II A (100% over 25bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35051782.7032739496752303Clontech SMART CDS Primer II A (100% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29195792.2516465225785565Clontech SMART CDS Primer II A (100% over 21bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19448631.4999231090652794Clontech SMART CDS Primer II A (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTG6751030.5206549719436779Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTA4940760.3810427829798484Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTG3630390.27998403259867144Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3445270.2657071521217348Clontech SMART CDS Primer II A (100% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3192700.2462283724001494Clontech SMART CDS Primer II A (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2673750.2062057539715286No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTA2656250.2048561136930801Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACCTTTTTTTTTTTTTTTTTTTTTTTT2304020.17769132539148438Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTC1999730.15422378022982136Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGG1859880.143438226347477Clontech SMART CDS Primer II A (100% over 26bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG1787750.1378753947312203Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1575920.12153857757786306Clontech SMART CDS Primer II A (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTAT1534960.11837964810327724Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1527700.11781974019347517Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA1427760.1101121373690097Clontech SMART CDS Primer II A (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTG1330320.1025973402986083Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)