FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_450D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_450D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30670676
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTTGATTGTAGATATTGGGCTG7182762.3418981700957615No Hit
AGAGTGGGTGTTGAGCTTGAACGCTT2682570.8746367377099872No Hit
AGTTGGTTGATTGTAGATATTGGGCT1718110.5601800234204163No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA1708280.5569750076587814No Hit
AGAGTGGGTATAATACTAAGTTGAGA1417630.4622102232112523No Hit
AGAGTGGGTACTTGCGCTTACTTTGT1231020.4013670908329507No Hit
ACTTGTTGGTTGATTGTAGATATTGG1075100.35053025893527745No Hit
ACACGACGCTCTTCCGATCTACACGA956300.31179619255865115No Hit
AGTGAGGGTAATAATGACTTGTTGGT861830.28099478472531875No Hit
TTTGGTAAACAGGCGGGGTAAGATTT820570.2675421956790258No Hit
GGTGAAGTCTTAGCATGTACTGCTCG742720.24215964460646386No Hit
CCTCTATATAAATGCGTAGGGGTTTT728940.23766675374223897No Hit
GTCAAGTGTTGCGCACGCCGCAAAAG692530.22579547969532854No Hit
GACGTAGGCTTATGCGGAGGAGAATG628020.20476235998189282No Hit
AGGGGTAAGATTTGCCGAGTTCCTTT625090.20380705009566794No Hit
AGAGGGTAATAATGACTTGTTGGTTG613460.20001515454044766No Hit
ATGCGATGTTGCCTCTACGCTCTTAC568460.18534315970081652No Hit
GTAACTGAGGATCGCACATCTCCACG529040.17249049222129959No Hit
GCAGTTACAGGTCCACAGTTAGCCGG466080.15196274121900671No Hit
AGGGATTTTTTAGGTAGTGGGTGTTG465430.1517508124046565No Hit
AGAGGGATATGAAGCACCGCCAGGTC441010.1437888098716833No Hit
TAGTTGGTTGATTGTAGATATTGGGC440470.1436127459336077No Hit
AGTTGGTCAAGTTATTGGATCAATTG430420.14033600041942343No Hit
CAGTCCTTTGAAGTATACTTGAGGAG410860.13395857332913041No Hit
TTGTAGGGGTTTTAGTTAAATGTCCT399860.130372085701665No Hit
GAAACTCGTAGCGCAAGCGGGCAGCG392130.12785176303254614No Hit
CACAGTACATACAGCTTAATGCCAGG390630.12736269653789176No Hit
GGGGTAGAAAATGTAGCCCATTTCTT385170.12558249449734984No Hit
AAGACCTAACGTGGGACTTCTCCTGA373150.12166344165351947No Hit
ATGAGGGTAATAATGACTTGTTGGTT364780.11893445061334808No Hit
ACAGCCGCAGACAAATCACTGCACCT364250.11876164711857018No Hit
ATCCACCTCTCGCATCGATAACGTCA363400.11848450943826605No Hit
TTTGGTTCATACTACGCTCTAATGGC342220.11157889053374631No Hit
ACTTTCATCTTGCCCTTCATTATTGC341760.11142891014205229No Hit
GAGTCCGCACGCGAAAATGTACTCGC341010.11118437689472511No Hit
CGACCTTGTCTAGGTTCCCTCCTCGT340420.11099201074016105No Hit
AGGGGTTTATCGATTACAGAACAGGC337420.1100138777508523No Hit
AACGTTGTCAATAAGGCCCACTACAC334510.10906508875122282No Hit
TCAACGAAGCGAGAAATTGGGTTTAC332240.10832496812264589No Hit
CTCGTACGTCCTGCTTGGTGCTTGGG330670.10781307852490764No Hit
CAGCACTCTACTCTCAGTTTACTGCT330660.10780981808160994No Hit
CTGCGGATCCCAGGTGCCCAGACGTA329370.10738922089620717No Hit
CCGGTAGTCTCCTATACCATCAGGTG327190.10667844425730948No Hit
CAAGATCGTGACGCCTATGGGTCTTG325280.10605569958744959No Hit
GACGTTAGTAGCTGCCCTCAGATGGA323750.10555685176290212No Hit
CGTCCTTTCGTACAGGGAGGAATTTG320550.10451350990763947No Hit
AAGTTGGACACGACTGAGTGACTTCA320220.10440591527881551No Hit
TAGCCGGGTTCCTTTTACTTTTTTTA319750.1042526744438238No Hit
AGCAGCCAGAGACGAAAACAGTCATT310550.10125306660994364No Hit
ATCCACCAGAACTGTAGACCGAGCTC310120.10111286754814273No Hit
TGAGCATTCCCAAGTACGTAAGCCAT309510.10091398050698329No Hit
CTTAACTTCCACTCCAACGTTTTCGG308310.10052272731125979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)