FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_450D0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_450D0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76010241
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGCGGGCTTATGCGGAGGAGAATG8298881.0918107732351487No Hit
GCGAGAACATTGTGCATCACTCCGGA3284340.4320917756332334No Hit
TAGTTGGTTGATTGTAGATATTGGGC3114460.4097421556655767No Hit
AGTTGGTTGATTGTAGATATTGGGCT3092200.40681360291963814No Hit
GGGTCTGTTAATTGTCAGTTCAGTGT3029050.39850551190858613No Hit
AACTTTCAGGCATGGTCCCCGTCCTC2607320.34302219881134177No Hit
TTGCGTCTCTTACCTAGGACCGGGGG2192260.288416399048123No Hit
CGTAGGACTTTAATCGTTGAACAAAC2182690.2871573581775645No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT2156010.28364730484146206No Hit
CCTCTATATAAATGCGTAGGGGTTTT1927140.25353688853584877No Hit
TAGTTGGGTTGACAGTGAGGGTAATA1574970.2072049738666136No Hit
ACGCCGAGTTCCTTTTACTTTTTTTA1511150.19880873683850048No Hit
CATGGCGGTATATAGGCTGAGCAAGA1318980.1735266172883204No Hit
GTCACGGTTAGGGTACCGCGGCCGTT1013710.13336492381335827No Hit
TGTTGGTTGATTGTAGATATTGGGCT1013080.13328204024507698No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT895630.1178301750154956No Hit
GATGTTCGCCGACCGTTGACTATTCT887000.11669480169126158No Hit
CTGGTCTGAACTCAGATCACGTAGGA855790.11258877603085089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)