FastQCFastQC Report
Wed 28 Aug 2024
HWTCTDMXY_n01_CITE-seq_test_TCells_410D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWTCTDMXY_n01_CITE-seq_test_TCells_410D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50398439
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTTAATTGTCAGTTCAGTGTTT3513900.6972239755282896No Hit
GTGTTGGGTTGACAGTGAGGGTAATA2984310.5921433399951137No Hit
ATCCACCTTCGACCCTTAAGTTTCAT2949700.5852760638082461No Hit
AAACGGGGTTTATCGATTACAGAACA1998120.3964646603439444No Hit
ACACGACGCTCTTCCGATCTACACGA1634420.3242997268228883No Hit
TGCTACCTTTGCACGGTTAGGGTACC1591080.3157002541289027No Hit
AAACGGGGGAAGGCGCTTTGTGAAGT1189410.23600135710552464No Hit
AGGGGTTTTAGTTAAATGTCCTTTGA1162170.23059642779809114No Hit
ACTTGTTGGTTGATTGTAGATATTGG1160240.23021347942939266No Hit
GCTGCTGCTGCTGCTGCTGCTGCTGC1022270.20283763153854825No Hit
TGGGGTTTTAGTTAAATGTCCTTTGA1013570.2011113875967468No Hit
GGGCTGTTAATTGTCAGTTCAGTGTT980600.19456951831385094No Hit
CCTCTATATAAATGCGTAGGGGTTTT978110.19407545539257676No Hit
AGTTGGTTGATTGTAGATATTGGGCT966570.19178570193414127No Hit
ATTGGGCTGTTAATTGTCAGTTCAGT942930.18709508046469456No Hit
GGGACCTAACGTCTTTACGTGGGTAC911310.18082107662104374No Hit
GGCTGGTTGATTGTAGATATTGGGCT906290.17982501402473994No Hit
GTGTTAGGTTGACAGTGAGGGTAATA880230.174654218953091No Hit
CTACATTCACAGGCCTTCATTTTGGA873950.1734081486134918No Hit
GGTGAAGTCTTAGCATGTACTGCTCG766710.15212971179524032No Hit
AGTGGGTATAATGCTAAGTTGAGATG765910.15197097672013216No Hit
GTTGGGTTGACAGTGAGGGTAATAAT763640.1515205659445127No Hit
ACTTATACTAACATTAGTTCTTCTAT758060.15041338879563312No Hit
AAACGGGGTTCGCTAATACAAGGTGG746740.1481672874828524No Hit
CAAGATCCACGCGAAATCACTGACTG726710.14419295803983137No Hit
TGGTTGATTGTAGATATTGGGCTGTT707790.1404388735135229No Hit
GACGCGGGCTTATGCGGAGGAGAATG691170.1371411523281505No Hit
TGGGCTGTTAATTGTCAGTTCAGTGT671920.1333215895833599No Hit
AACTCTTTCCTTGACCTTCGATCACG666280.13220250730384725No Hit
AAGACCTAACGTCTTTACGTGGGTAC659660.13088897455732706No Hit
GATGTTCGCCGACCGTTGACTATTCT658720.13070246084407494No Hit
ATGAGGGTAATAATGACTTGTTGGTT655620.13008736242803076No Hit
TTAGTTCTTCTATAGGGTGATAGATT652700.1295079794038859No Hit
GTCTCCTCTATATAAATGCGTAGGGG639220.1268332933883131No Hit
TAGTTGGTTGATTGTAGATATTGGGC634030.12580349958854878No Hit
ATTGGGTGTGAGGAGTTCAGTTATAT625700.12415067061898484No Hit
AAGACCTTCTAGCACAACCCCTCCTC608620.12076167676542521No Hit
GGGGTAGAAAATGTAGCCCATTTCTT604820.12000768515866138No Hit
TGTTGGTTGATTGTAGATATTGGGCT603530.11975172485004942No Hit
AGTGGGTGTTGAGCTTGAACGCTTTC596430.11834295105846433No Hit
GGCTGCACCATCGGGATGTCCTGATC590250.11711672260325365No Hit
GGGCCAGCCAGGCAACCTTCTAGGTA581160.11531309531233695No Hit
GTGTTGGTTGATTGTAGATATTGGGC580120.11510673971469633No Hit
GGGACCTGCTAAACCTAGCCCCAAAC568410.11278325505280035No Hit
GTGATCTCCTCGAGTCCCACACCTGC559640.11104312179192692No Hit
GGCGTGTCACGAGGTAGCAATGGGGC559620.11103915341504922No Hit
GTTCGGGGTTTATCGATTACAGAACA554130.10994983396211934No Hit
TGTGTTTTAATCTGACGCAGGCTTAT547800.10869384268032588No Hit
GTTCGGGCATTGGGCCCGACAACGGA544870.10811247546774216No Hit
TTCTCCTCTATATAAATGCGTAGGGG541300.10740412019507191No Hit
AGCTCTCTCACTATTCTTTGTTGGTC540140.10717395433616506No Hit
TCAATCTAGTCTCCTCCTTTATCTCG538940.10693585172350278No Hit
CACAAACTCAAACGGGCTAAAGTCTT533860.10592788399656586No Hit
AACTTTCACGCATTGGAGAAGGAAAT533500.10585645321276717No Hit
CGGCTGTTAATTGTCAGTTCAGTGTT533280.10581280106711241No Hit
AGCCTTCATCAGGGTTTGCTGAAGAT529320.10502706244532693No Hit
CAAGTTGAGTCTCCTCTGACTCGGCC522670.10370757713349019No Hit
GCAGTTAGTTCCACTCTTGGGCGCCG512560.10170156262181056No Hit
GCACTCTTCATCTGTTCTTCGACCAT510880.10136821896408339No Hit
GGCTGGTTTTAGGCCTACTATGGGTG510520.10129678818028472No Hit
CACCACTGTCAGTGGACCCCGTGCAA504960.10019357940828286No Hit
GCAGTTATATGTTTGGGATTTTTTAG504520.10010627511697337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)